Site-Directed Mutagenesis

Site-directed or oligonucleotide-directed mutagenesis is a key method in the study of protein structure/function and gene expression control. If the nucleotide sequence of the gene is known, an oligonucleotide can be manufactured to introduce a single base change in the codon corresponding to the specific amino acid to be altered. The oligonucleotide is then used to generate a set of clones that can be identified and propagated. Learn More.

The Trimer Oligo Solution

While randomers are often used in protein mutagenesis procedures, they have several short comings. First, it is very difficult to achieve a truly random mixture. The second and more problematic issue is the inevitable introduction of stop codons. Fortunately, the ability to direct combinatorial mutagenesis using randomized oligonucleotides has been advanced by the use of trimer oligonucleotides. Read More.

Incorporating 2′ Modified NTPs

Wondering which RNA polymerase to use for incorporation of 2′ Fluoro and 2′ O-Methyl NTPs? We recommend T7 R&DNA™ and SP6 R&DNA™ polymerases from Epicentre. We’d like to hear from you – click the link below to leave your comment.

Have a Question?

Collectively the TriLink Team has many years of experience in nucleic acid chemistry to offer. Post your question in the comment field below and we will get an answer to you as soon as we can.

Long Oligonucleotides

Thank you Prithwish for your most recent question regarding the synthesis of very long oligonucleotides. We know research is in need of longer and longer synthetic oligonucleotides and have been working to push through current synthesis barriers. Thus far, we have succeeded in producing high quality DNA 180mers and RNA 100mers. We are continuing to push these limits. Learn more in our January Newsletter.

View Prithwish’s question and our answer here.

Publication Reprints

Reprints of TriLink’s publication “3’-Protected 2’-Deoxynucleoside 5’-Triphosphates as a Novel Tool for Heat-Triggered Activation of PCR” are now available. To request a copy, please email info@trilinkbiotech.com.

Click here for more TriLink Publications.

Ask An Expert!

Welcome to our new Ask An Expert blog.  Answers to frequently asked questions  are listed by topic on the Ask An Expert homepage.  Please feel free to ask an expert by clicking on the comment link below. Thank you!